Mother to fry, successful transfer of immunity against infectious haematopoietic necrosis virus infection in rainbow trout.
نویسندگان
چکیده
We have tested whether immunity can be transferred from a mother fish to its fry. Rainbow trout mother fish were inoculated against infectious haematopoietic necrosis virus (IHNV) by intraperitoneal injection of a fragment of the IHNV glycoprotein spanning amino acids 31 to 310. This protein fragment was obtained by isolating the specific cDNA from Japanese IHNV strain HV7601 and expressing it in Escherichia coli. Fry from immunized and control fish were exposed to IHNV at various intervals after hatching, and their mortality monitored. Survival of the fry of immunized fish was significantly greater when exposure to virus occurred 7 days after hatching, and some immunity appeared to persist until at least 25 days after hatching.
منابع مشابه
Isolation and Identification of the Infectious Haematopoietic Necrosis Virus (IHNV)-like Agent from Farmed Rainbow Trout (Oncorhynchus mykiss) from Iran
متن کامل
بررسی آلودگی به ویروس IHN در مزارع پرورش ماهی قزل آلای رنگین کمان (On corhynchus mykiss) در دو استان تهران و چهار محال و بختیاری با استفاده از تکنیک آنتی بادی درخشان به روش غیر مستقیم
Objective: Serological diagnosis of infectious haematopoietic necrosis virus (IHNV) in tissues of kidney, spleen and liver of rainbow trofi(O.mykiss)fry. Design: Cross- sectional study. Animals:Ninty nine samples consisting of smears from kidney, spleen and liver were obtained from rainbow trout. Procedure: Kidney, spleen and liver from rainbow trout (O.mykiss) fry less than 3 g body weight obt...
متن کاملPhylogenetic relationships of Iranian Infectious Pancreatic Necrosis Virus (IPNV) based on deduced amino acid sequences of genome segment A and B cDNA
Infectious Pancreatic Necrosis Virus (IPNV) is the causal agent of a highly contagious disease that affects many species of fish and shellfish. This virus causes economically important diseases of farmed rainbow trout, Oncorhynchus mykiss, in Iran which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout fry were collected during...
متن کاملIn vivo effect of EICAR (5-ethynyl-1-b-D-ribofuranosylimidazole-carboxamide) on experimental infected rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch) fry with infectious pancreatic necrosis virus
The in vivo antiviral effect of 5-ethynyl-1-b-D-ribofuranosylimidazole-carboxamide (EICAR) was evaluated in coho salmon and rainbow trout fry, experimentally infected with infectious pancreatic necrosis virus (IPNV). Treatment consisted of a daily bath of 2 h in 0.4 mg ml or 0.8 mg ml of EICAR, for approximately 20 days. The behavior of the fish was studied for 45 days post-infection. The survi...
متن کاملPhylogenetic relationships of Iranian Infectious Pancreatic Necrosis Virus (IPNV) based on deduced amino acid sequences of genome segment A and B cDNA
Infectious Pancreatic Necrosis Virus (IPNV) is the causal agent of a highly contagious disease that affects many species of fish and shellfish. This virus causes economically important diseases of farmed rainbow trout, Oncorhynchus mykiss, in Iran which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout fry were collected during...
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عنوان ژورنال:
- The Journal of general virology
دوره 77 ( Pt 10) شماره
صفحات -
تاریخ انتشار 1996